THE TRANSFORMATION OF NARRATIVE: AN EXPLORATION OF ALLITERATION'S FUNCTION IN VLADIMIR NABOKOV'S AUTOBIOGRAPHY
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The following is a psychoanalytic inquiry that uses the successive publishings of Vladimir Nabokov's autobiography to explore the functions that various versions of repetition play in transforming traumatic or conflicted memories into narratives that represent more symbolized and coherent accounts of the self. The data set consists of the three, successive, published, English language versions of Nabokov's memoirs. These successive versions are useful in that they represent Nabokov's repeated return to the significant memories and material that compose his autobiographical narrative. With each returns, Nabokov made meaningful revisions to his narrative. Consequently, his transformations are tracked over the course of the successive, drafted versions. Specifically, this project focuses on one chapter of Nabokov's autobiography--"Mademoiselle O"--she being a figure who loomed large in the early part of Nabokov's life. This project proposes that repetition manifests in any one of three main forms: as static repetition, as means for transformation, or as a means of creative play. In addition it proposes that these forms of repetition correspond to a continuum of symbolization, with desymbolized stasis on one end and symbolized play on the other. Here, alliteration was identified as a signifier for these varied forms of repetition. Two main hypotheses are proposed. The first states that if alliterative repetition marks emotionally salient material, then the concentration of alliteration in Nabokov's autobiographical narrative would be greater in areas that contain his most conflicted themes--loss and exile. Indeed results reveal significance. The second hypothesis proposes that if alliterative repetition serves a transformative function, then the concentration of alliteration would decline over the course of Nabokov's progressive drafts. Results are not significant but do reveal a trend toward increase in the final draft. The discussion explores various possibilities as to the given results. It is suggested that Nabokov may have increased his use of alliterative repetition because of the pleasure derived out of mastery and play. Alternatively, it is suggested that alliteration might facilitate internalization, in this case where reader internalizes author and work, thus resulting in Nabokov being better remembered by his readers, an elegant solution to his concerns around loss and being lost.
Respecting Hair: The Culture and Representation of American Women's Hairstyles, 1865-90
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Respecting Hair: The Culture and Representation of American Women's Hairstyles, 1865-90 by Elizabeth L. Block Adviser: Professor Kevin D. Murphy Using a hybrid approach that merges art historical and material culture inquiry, this dissertation recognizes the centrality of hairstyles in figure painting, both portraiture and genre, and photography of the mid- to late nineteenth century in the United States. After establishing the pervasive reach of hair's culture and industry (Chapter One), it argues that artists exploited women's hairstyles as a way to convey commentaries on such topics as conspicuous consumption and monetary wealth (Chapter Two), social class and the development of the modern woman (Chapter Three), the New Woman (Chapter Four), publicly exposed women "à la toilette" and "en déshabillé" (Chapter Five), and overt sexuality (Chapter Six). It considers the specific ways in which artists depicted hair and how that treatment helped achieve their goals. It affirms that hair deserves serious attention with regard to its cultural significance, specifically within the American art historical context of the nineteenth century, which has not been addressed in any publication to date. The study begins in the mid- to late 1860s with the considerable rise in new advertising, products, and services related to hair after the Civil War and how these phenomena were treated by artists. It proceeds to discuss the entrenchment of the Cult of True Womanhood of the 1860s and 1870s, which had a patriarchal and conservative effect on hairstyles and their depiction in art. The emergence of the New Woman, which brought about a radical consideration of hairstyles about 1890, provides an end point. By tracing the development of women's hairstyles, this dissertation contends that the study of hair should take its place with readings of other visual culture in paintings, such as clothing, furniture, and interior decoration that broaden our view into the motivations behind cultural changes. The study highlights the following artists: Aaron Draper Shattuck (1832-1928); Thomas Pritchard Rossiter (1818-1871); Eastman Johnson (1824-1906); Winslow Homer (1836-1910); Alice Austen (1866-1952); Mary Cassatt (1844-1926); and John Singer Sargent (1856-1925).
Unemployed and Poor in New York: The Impact of Object Relations, Mentalization and Psychopathology on Job Outcome
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This study examined the relationships between the quality of internal object representations of self and other (OR), the capacity for reflective functioning (RF) and the presence of Axis II psychopathology and their respective and combined impact on the ability of unemployed, low-income individuals to complete job readiness training, and to obtain and retain employment. Given the intertwining nature of these constructs, it was expected that correlations would exist between OR, RF and Axis II psychopathology and that these constructs would also be related to job outcome, such that those with low OR and RF or those with Axis II psychopathology would experience greater difficulty in completing job training, let alone obtaining and maintaining employment. This research study posed additional research questions to examine the extent to which each of these variables would account for the variance in job outcome. It also sought to investigate the extent to which the predicted relationship between OR and job outcome would be moderated or mediated by Axis II pathology or the degree of RF present, such that an individual with significant psychopathology or low RF capacity would be expected to have poor job outcome regardless of OR scores. Similarly, it examined the question of whether the proposed relationship between Axis II pathology and job outcome would be moderated or mediated by the degree of RF present, such that those with a more developed capacity for RF would have better job outcome despite the presence of Axis II pathology. Results partially supported the study's main hypotheses in that RF and Axis II pathology were not only found to be related, but also to significantly predict job outcome. Moreover, it was determined that in those cases where participants with Axis II diagnoses were able to obtain jobs, their ability to obtain the job was entirely attributable to the presence of relatively higher levels of RF. This finding suggests that the presence of even a moderate capacity to consider and to reflect upon the mental states of self and other confers an advantage on those with Axis II diagnoses in the pursuit of gaining employment. OR findings were less robust although one of the subscales of OR, Complexity of Representations, was found to be significantly associated with RF in the predicted direction. These results are discussed in relation to implications for the design of programs and interventions to assist unemployed and underserved populations.
DNA Adducts of 10-decarbamoyl Mitomycin C Activate p53-dependent and p53-independent Cell Death
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Mitomycin C (MC), a natural antibiotic and DNA cross-linking agent, has cytotoxic activity and is known to activate the tumor suppressor p53 protein. 10-decarbamoyl mitomycin C (DMC), a derivative of MC, has increased cytotoxicity compared to MC. Both MC and DMC induce cellular cytotoxicity in cells with wild-type p53, while only DMC shows significant cell death activity in the absence of wild-type p53. We investigated the difference in MC and DMC cytotoxicity by comparing DNA adduct composition and the cellular regulation of molecular targets in human cancer cell lines with or without wild-type p53. Compared to MC, DMC produced substantially more mitosene-1-β mono and 1-β cross-link adducts in DNA and resulted in abnormal nuclear morphology in human cancer cells with or without p53. Significantly, greater poly(ADP-ribose)polymerase (PARP) activity was observed after DMC treatment in both the presence and absence of wild-type p53. Both MC and DMC induced double strand breaks as indicated by gamma-H2AX foci formation irrespective of the p53 status, suggesting that double strand breaks cannot account for DMC's increased cytotoxicity. In cell lines expressing wild-type p53, both MC and DMC signaled for p53 stability and apoptosis induction resulting in cleavage of procaspase-3 and -8. Despite the DMC induced cellular cytotoxicity observed in cell lines lacking wild-type p53, cleavage of procaspase-3 or -8 was not observed in these cells. However, we observed an increase in caspase activity. Caspase-2 activation has been suggested as a pathway for p53-independent cell death in the absence of Chk1. Interestingly, Chk1 was depleted following DMC, but not MC treatment in cells with or without wild-type p53. This Chk1 depletion was achieved through the ubiquitin proteasome pathway since chemical inhibition of the proteasome protected against Chk1 depletion. Additionally, gene silencing of Chk1 by siRNA increased the cytotoxicity of MC but not of DMC. DMC treatment also caused a decrease in the level of total ubiquitinated proteins without increasing proteasome activity. This suggests that DMC- mediated DNA adducts facilitate signal transduction to a pathway targeting proteins for proteolysis. In conclusion, we have found that DMC generates significantly more mitosene-1-β stereoisomeric DNA adducts than MC and causes rapid down-regulation of multiple cellular targets. These studies suggest increased mitosene-1-β stereoisomeric DNA adducts more effectively signal for a mode of cell death which does not require a functional p53 protein.
The Value of Diversity: Culture, Cohesion, and Competitiveness in the Making of EU-Europe
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This dissertation examines a particular way of governing (through) "culture" as a means to reflect on the making of a "non-national" state form (EU-Europe) and its implications for social inequalities. The term EU-Europe highlights complex and often conflictual relations between European Union (EU), national, and regional governmental levels, and the study focuses on relations between the EU and the city of Berlin. The dissertation critically examines the development of a policy common sense that emphasizes the potential value of cultural diversity for economic competitiveness. Such value, it is assumed by policy-makers, can be realized by combining support to the creative and cultural industries with an approach to immigrant integration that respects individual cultural diversity, ensures equality of opportunity, and fosters intercultural dialogue. Because such interculturalism goes on the EU level hand in hand with a new narrative of EU-Europeanness, the study also "moves outwards" onto relations of EU accession established with Turkey where this has been articulated particularly clearly. The study argues that interculturalist policy constitutes an attempt to overcome challenges to legitimacy and cohesion on EU and city levels by establishing "non-national" modes of belonging and entitlement that work with the neoliberal agenda that has dominantly informed EU-European state-making of the last decades. In selectively embracing cultural diversity, such policy is to turn "culture" from a problem into a resource in the making of "cosmopolitan" places conducive to capital. In Berlin, this has fed into processes of gentrification that serve the generation of rent and effectively void the "right to place" of populations marked through class and culture. In the politics of Turkey's EU accession, the claim that EU-Europeanness is defined through an embrace of diversity has in turn obscured and enabled EU support of the development of a Turkish "competition state." The dissertation furthers our understanding of contemporary "non-national" forms of statehood and of the ways in which these (re)produce inequalities between people and places. It is based on extensive analysis of policy and political documents, interviews with key policy-makers, attendance of policy events, and experience of local politics in Berlin gained during a stay of 12 months.
Parental Investment and Song Learning in Zebra Finches (Taeniopygia guttata)
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Abstract PARENTAL INVESTMENT AND SONG LEARNING IN ZEBRA FINCHES (TAENIOPYGIA GUTTATA) By DIANE M BOGDAN Adviser: Professor Cheryl F. Harding In order to understand the effects of parental investment on learning, we conducted a series of experiments using zebra finches. The aspects of parental investment studied included deposition of maternally-derived hormones (MDH) into eggs, feeding and attention to chicks. Learning was assessed by song copying (mean accuracy, sequential matching and percent similarity to the father's song). We compared digit ratios, a marker for the amount of MDH chicks experienced in eggs, and song copying ability. Our data suggests that maternally-derived testosterone negatively affected the ability to sequentially match notes. To determine if parents were preferentially feeding chicks by hatch order, we weighed chicks at key developmental points prior to fledging. We found that chicks that hatched early were heavier than those who hatched late. Additionally, weight at day 10 was positively correlated with song learning. Acoustic cues are one obvious way that parents might differentiate chicks by hatch order. Therefore, we assessed the begging calls of 10 day old chicks. We found that early-hatched chicks begged at lower amplitudes than late-hatched chicks. We then conducted a playback experiment in which begging calls of 10 day old early- and late-hatched chicks were presented to breeding adults. Adults were more attentive during the early-hatched chicks' playbacks. To determine if attention, in the form of clumping or perching closely together, affected song learning, we observed family groups when chicks were beginning song acquisition (day 25). We found that while clumped with their mates, mothers clumped with first-hatched more than second- or third- hatched chicks. Moreover, clumping behavior was positively correlated with the percent similarity of song copying. Clumping with first-hatched sons may be a way for mothers to give additional access to the father and thereby enhance son's song learning. Finally, we used a multiple linear regression, combining all three forms of parental investment to determine which were more important in song learning. We found that digit ratio 2:3 was positively correlated with sequential matching, nestling weight positively correlated with mean accuracy and clumping with the mother positively correlated with the percent similarity score.
PREDICTING INTRODUCTIONS AND RANGE EXPANSIONS OF THE MONK PARAKEET WITH ECOLOGICAL NICHE MODELING AND LANDSCAPE GENETICS
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The ability to predict species future geographic distributions is an important challenge in biogeography and conservation biology, with critical implications for pressing environmental issues, including the potential spread of invasive species. This research examines a two-step framework to build accurate predictions of the invasive potential of the monk parakeet (Myiopsitta monachus). This species, native to temperate South-America, has established several stable populations worldwide, and shares many of the typical traits of high-risk invaders. The proposed framework aims to 1) identify areas where the species is likely to thrive, and 2) determine which of these suitable areas the species can likely disperse to. Objective 1 requires identifying the environmental conditions suitable to the species, for which I used ecological niche modeling (Chapter 1 & 2). Objective 2 addresses the ability of the species to conquer new adjacent favorable areas via dispersal, a problem that can be addressed with landscape genetics (Chapter 3). In Chapter 1, I developed a null model approach to evaluate the performance and significance of ecological niche models. The results highlight the importance of accounting for both discrimination and overfitting and correctly estimating significance. In Chapter 2, I tested the effect of different model calibration strategies on transferability (the ability to predict independent data in different geographic regions). I used this information to make predictions about the global invasive potential of the monk parakeet. The best prediction was obtained with native calibration records and complex model settings. This prediction indicates several areas with conditions suitable for monk parakeets, including areas adjacent to existing introduced populations. In Chapter 3, I integrated ecological niche modeling and landscape genetics to make predictions about the landscape features that affect monk parakeet dispersal. I tested these predictions with genetic data from an introduced population in Florida, and assessed their significance with null models. Estimating resistance to dispersal with ecological niche modeling produced results equivalent to evaluating a range of alternative hypotheses with a stepwise regression model. The results indicate that monk parakeet may not be limited by distance and most landscape features and are likely to expand to adjacent suitable areas.
The Characterization of Black Inkjet Computer Printer Inks using Pyrolysis Gas Chromatography-Mass Spectrometry (Py-GC-MS), High Performance Liquid Chromatography (HPLC), Thin Layer Chromatography (TLC) and Attenuated Total Reflection Fourier Transform Infrared Spectroscopy (ATR FT-IR)
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Documents are prevalent in every aspect of daily life and hardly a day passes without using some sort of document. Problems arise, however, when the authenticity of these documents is raised. Forensic science has long been involved in the investigation and examination of suspect documents. One of the steps in the examination of questioned documents is for the examiner to analyze the type of material used to create the document. This could involve the analysis of the paper substrate and/or the medium used to create the written word, namely pen ink, typewriter ink or toner in photocopied documents. This is the age of the computer, and as a result new challenges are facing the questioned document examiner. With more and more individuals using computers to produce their documents, and with the advancement of more sophisticated computer and printer systems, it has become harder for the analyst to distinguish and possibly individualize a suspected document based on physical appearance alone. Once again, the forensic scientist must focus on the material used to produce the document, namely the computer printer ink. An examination of these ink samples may allow for the differentiation between the many manufacturers, as well as within the products of a specific manufacturer. In time, it may also be possible to date a computer printer generated document based on the drying and decomposition rates of the different computer printer ink components. Currently, this is unfortunately still just a theory. There have been few studies on the different types of computer printer inks and how, or if, they differ from each other. The identification of the various black inkjet computer printer ink manufacturers, and the creation of a classification procedure, is the first step in the analysis of a questioned inkjet produced document. The goal of this study was to produce a detailed document on the forensic identification of black inkjet computer printer inks, and this research succeeded in its goal. The analysis of the black inkjet computer printer ink samples by Thin Layer Chromatography (TLC), High Performance Liquid Chromatography (HPLC), Pyrolysis Gas Chromatography-Mass Spectrometry (Py-GC-MS) and Attenuated Total Reflection Fourier Transform Infrared Spectroscopy (ATR FT-IR) analytical methods resulted in the production of data that led to the establishment of a classification procedure that could assist the forensic scientist in the examination, identification and discrimination of the different inkjet computer generated documents they receive.
CANDIDA ALBICANS ALS5P AMYLOID IN HOST-MICROBE INTERACTIONS: A CEANORHABDITIS ELEGANS STUDY
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Candida albicans, a dimorphic fungus and an opportunistic pathogen, possesses a myriad of adherence factors including members of the agglutinin-like sequence (Als) family of mannoproteins. The adhesin Als5p mediates adhesion to many substrates, and is upregulated during commensal interactions, but is downregulated during active C. albicans infections. An amyloid forming core sequence at residues 325-331 has been shown to be important for Als5p function, because a single amino acid substitution at position 326 (V326N) greatly reduces Als5p-mediated adherence. We evaluated the role of Als5p in host-microbe interactions, using Caenorhabditis elegans as a host model and feeding them Saccharomyces cerevisiae expressing Als5p on the surface. Als5p-expressing yeast had increased intestinal accumulation rates when compared to non-expressing S. cerevisiae or yeast expressing the amyloid deficient Als5pV326N, respectively. Surprisingly, this accumulation delayed S. cerevisiae-induced killing of C. elegans. Treatment with the amyloid-inhibiting dye Congo red or repression of Als5p expression abrogated the protective effect of Als5p. Being that reproductive fitness is the most important measure of a pathogen's impact on the host, we looked at oocyte quantity and quality. Als5p had no effect on oocyte quantity or quality. In order to understand why nematodes exposed to Asl5p were able to harbor the cells expressing functional amyloid, we looked into the innate immune system of the nematode. Toll- Like receptors (TLRs) are important mediators of innate immune responses to Candida albicans, and several classes of scavenger receptors have been implicated in recognizing and reacting to a variety of ligands in humans. The C. elegans genome encodes for a single TLR, TOL-1, and scavenger receptor CED-1[4,5]. CED-1 is the orthologue to mammalian scavenger receptor SCARF1 and is required for defense against Cryptococcus neoformans[4,6]. Our studies showed that CED-1 was necessary for prolonged survival in the presence of Als5p, and TOL-1 was required for death in C. elegans fed S. cerevisiae. We have further demonstrated the necessity of CED-1 and TIR-1 in phosphorylation of ERK-2/MPK-2. The SAM and TIR domains of TIR-1 were shown to be necessary in discriminating the presence of functional amyloid, and thus elicit specificity in downstream signaling. Remarkably, the presence of the HEAT/Armadillo domain, alone, was sufficient to increase levels of phosphorylated ERK-2/MPK-2 in nematodes fed any yeast strain in this study. This study is the first to show that expression of amyloid-forming Als5p in S. cerevisiae can: a) attenuate S. cerevisiae pathogenicity in C. elegans; b) move the yeast-host interaction towards hallmarks of commensalism; c) be discriminated against by host pathogen recognition receptors (PRRs) leading to a slower decline in viability; and d) can lead to distinct downstream MAPK responses.
The Interchange of Plain Velar and Aspirate in Kronos/Chronos: A Case for Etymological Equivalence
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Despite the current state of uncertainty regarding the etymology of Kronos, the equivalence long familiar to the ancients between Kronos and Chronos is still a moot point. Arguments denying their etymological equivalence can no longer firmly rely on linguistic arguments. It is therefore necessary to examine the validity of the time-honored interpretation of Kronos as the personification of Time. The solution to this problem is of considerable importance to Classical studies, since it will not so much as contribute to a better understanding of the myth of Kronos, as the interpretation of Kronos as Time is already familiar from ancient sources, but it will demand a rereading of Hesiod's Theogony to account for the possible relation of its myth and symbols to comparable myths and symbols of the transitioning ages of the world and consequent calendrical corrections.