Professor Hyungsik Lim is interested in understanding neurodegenerative disorders from a biophysical perspective, using multiphoton microscopy (MPM) as the primary tool for imaging the intravital mouse brain (Lim, Front. Mol. Bio. 2019). Innovating advanced imaging modalities has been the theme of his research since postdoctoral training. While at the Wellman Center for Photomedicine, Lim demonstrated novel swept-source optical coherence tomography (OCT) for imaging the human retina in vivo, achieving the record for the highest speed (Lim et al., Opt. Express 2006). In the laboratory of Dr. Watt W. Webb at Cornell University, where MPM was invented, he studied the fundamental limits in the miniaturization of MPM toward clinical endoscopy. Now in his lab at CUNY Hunter College, Lim and his colleagues study novel concepts for elucidating neurodegenerative disorders including glaucoma. It was also in the Webb lab that his research interest on single-molecule biophysics began. Recently during a sabbatical year 2017-2018, Lim was a visiting associate professor in the laboratory of R. H. Singer working on intravital imaging of single-molecule gene expression, which he continues at CUNY.